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Jurnal Agroekoteknologi

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Published by Universitas Sultan Ageng Tirtayasa

ISSN : - EISSN : 25487108 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Journal of Agroecotechnology contains articles directly sourced from the results of research related to the cultivation of plants related to soil, plants, weeds, pests and diseases.

Arjuna Subject : -

Articles 10 Documents

Search results for , issue "Vol 1, No 1 (2009)" : 10 Documents clear

PENYISIPAN GEN FITASE PADA TANAMAN TEBU cv. PA 175 MELALUI Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC) Susiyanti .; Gustav Adolf Wattimena; Memen Surahman; Agus Purwito; Dwi Andreas Santosa
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACT Sugarcane (Saccharum officinarum L.) is one of importance crop grown in marginal in Indonesia. Phosphorus (P) is critical to the growth and development of plant in the marginal land. P is stored in plant as phytic acid (myo-inositolhexakisphosphate). Phytic acid is hydrolyzed by the activity of phytases to yield inositol and free phosphate. Genetic transformation of sugarcane with phytases gene holds promise to provide enough P during period of rapid cell division and growth of plant. Plant transformation mediated by Agrobacterium tumefaciens, has become the most used method for the introduction foreign genes into plant cells and the sub sequens regeneration of transgenic plant. The selection and regeneration of embryogenic callus of transformed plant was done on MS medium containing kanamycin. The main objective of this study were: (1) To find the best kanamycin concentration for selectable marker; (2) Insert phytase gen into varieties of sugarcane (cv. PA 175) through Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC); and (3) To analyze of intregated transgene into genom of sugarcane using PCR method. Result of the experiment showed: (1) kanamycin selectable marker lethal doses for transformed sugarcane calli: 100 mg l-1; (2) Efficiency transformation of putative transgenic line was cv. PA 175= 24 %; (3) The first culture of transformed calli become 24 (Triton cv.), 18 (PSJT 94-41 cv.), and 30 (PA 175 cv.) putative plants; the second sub cullture of putative eksplant regenerate become new plant: 380 (PA 175 cv.) plants. (4) Analyzed of integrated phytase gene was proven by appearance of 900 bp of PCR band (5) transgenic plants (cv. PA 175) with highest activities respectively: 45 %; with medium phytase activity: 27 %, and low phytase activity: 27 % from total of sample. Non transgenic plants, most of sample show low phytase activity respectively: 100 % , noneh show medium - hight activy of phytase. Key words: Sugarcane, transformation, phytase, Agrobacterium tumefaciens

PERFORMANCE OF PB 330 CLONE RUBBERS Yardha .
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTKeragaan Klon Karet PB 330 bertujuan untuk mengevaluasi tingkat adaptasi terhadap lingkungan, pertumbuhan tanaman, produktivitas lateks, mutu lateks dan sifat klon karet PB 330, potensi hasil kayu serta ketahanan terhadap hama dan penyakit. Evaluasi adopsi dan keragaan klon karet PB 330 dilakukan melalui pengumpulan data primer, data sekunder dan wawancara dengan peneliti maupun pelaku pengembangannya dengan menggunakan kuesioner terstruktur. Data sekunder diperoleh dari Perusahaan Perkebunan dan Tinjauan Pustaka. Data primer diperoleh melalui kunjungan lapang (field assessment) ke kebun produksi dan kebun pembibitan yang dilakukan pada bulan Oktober 2007. Evaluasi mutu hasil dilakukan melalui studi pustaka dan kunjungan ke pabrik pengolahan. Data hasil survey dianalisis secara deskriptif dan ditampilkan dalam bentuk tabel maupun gambar. Klon PB 330 ternyata memiliki pertumbuhan dan produksi yang sangat baik, dapat dipanen pada umur 4 tahun dengan produktivitas yang langsung tinggi (quick starter or precocious yielder). Dibanding klon PB 260 yang merupakan klon terbaik saat ini, klon PB 330 ternyata memiliki produktivitas sekitar 11% lebih tinggi. Dengan pertumbuhan lebih tegap, maka diperhitungkan PB 330 juga memiliki potensi produksi kayu yang tinggi, mempunyai daya adaptasi cukup baik terhadap lingkungan pertanaman karet di Indonesia dan belum ada laporan kendala gangguan hama dan penyakit yang serius di pertanaman komersial.Kata kunci: PB 330. klon karet unggul, keragaan, produksi.

APPLICATIONS OF ORGANIC FERTILIZER AND SEED TREATMENT OF CABBAGE IN LOWLAND AREAS JAMBI PROVINCE Salwati .; Yardha .
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTPermintaan terhadap sayuran organik telah meningkat pesat sejalan dengan pekembangan informasi dan pengetahuan tentang perlunya mengkonsumsi makanan sehat. Teknologi pertanian organik baru saja diadopsi petani sayur di kota Jambi. Karena itu, petani sayur memerlukan bimbingan melalui percontohan dan penyampaian informasi teknologi. Riset aksi tentang sistem pertanian organik tanaman selada dilakukan di kota Jambi dari September hingga Februari 2007. Beberapa paket teknologi telah diterapkan dan diuji yaitu: perlakuan benih (tanpa dan dengan Nutrifam SD), pupuk organik (Bio-super active, Jus Bumi, Nutrifarm AG, Botani and Bokasi+manure+botani). Percobaan ditata dalam Rancangan Acak Kelompok dengan perlakuan faktorial dan pengaruhnya dianalisis dengan uji beda nyata Duncan (DNMR). Parameter pengamatan meliputi jumlah daun, luas daun, lebar tanaman, tinggi tanaman, biomassa tamanan, dan tingkat serangan hama dan penyakit. Hasil penelitian menunjukan bahwa perlakuan benih tidak berpengaruh nyata terhadap pertumbuhan tanaman. Pupuk organik bitani memberikan pengaruh nyata terhadap bobot tanaman (1,45 kg), tinggi tanaman (15,73 cm), luas daun (57,94 cm2) dan tingkat serangan hama/penyakit (8,67%. Sistem pertanian organik memiliki prospek sangat cerah untuk dikembangkan.Kata kunci: Selada, Propinsi Jambi , sistem pertanian organik

PENGENDALIAN PENYAKIT LAYU FUSARIUM PISANG (Fusarium oxysporum f.sp.cubense) DENGAN SOLARISASI TANAH DAN BAKTERI ANTAGONIS Andree Saylendra
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTThe objective of the research is to investigate the effectiveness of soil solarization and antagonist bacteria in controlling fusarial wilt of banana.The experiment was conducted in farmer field by randomized complete block of two factors. The treatment in the field, factor A is solarization (without solarization, three weeks of solarization, and four weeks of solarization); factor B is antagonist bacteria (without bacteria, bacteria 1, and bacteria 2). The treatment is replicated three times. The number of banana’s plant for each treatments are 4-6 plants. The result of field research showed that single treatment of solarization, bacteria even combination between them did not significantly affect to suppress the incident of Foc.Keywords: Fusarium oxysporum f.sp.cubense, soil solarization, antagonist bacteria

OPTIMASI METODE ISOLASI DNA GENOM PADA TANAMAN KAPULASAN Ediwirman Ediwirman; Ellina Mansya
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTHigh quality and quantity of genomic DNA for plant kapulasan is required for analyses based on the polymorfisme chain reaction (PCR) technic. Method DNA isolation were proven to be genus or even species spesific. Objective of this study was to determine of methode with using protocol most reliably produced sufficient quantity and good quality of plant kapulasan genomic DNA. Method utilizing Doyle and Sanghai-Maroof with storge of leaf until dry, yielded higher quality and quantity of genomic DNA.Key words : DNA, Kapulasan

COST-BENEFIT ANALYSIS PENGELOLAAN SUMBERDAYA PESISIR TELUK BANTEN BERKELANJUTAN Sjaifuddin .
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTBanten bay coastal zone is a unique ecosystem which has a variety potencies and problems of using some natural resources, especially in the trade off between economic growth and ecological preservation. Based on these conditions, this research aimed to identify the management feasibility of Banten Bay coastal resources. An extended cost-benefit analisys which integrated the external and/or environmental benefits, damage cost and cost of society of environmental protection measures was used as methods of this research. The result of this research showed that all of management options of Banten Bay coastal resources were feasible to be developed. Based on comparative performance index, it was known that sustainable mangrove management, sustainable harvest and beach protected areas were the most feasible management option to be implemented in the management of Banten Bay coastal resources. Key words: cost-benefit analysis, sustainable management, coastal and marine resources.

PERBAIKAN PENAMPILAN TANAMAN KRISAN DENGAN INDUKSI MUTASI Yusniwati PK. Dewi Hayati .; PK. Dewi Hayati
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v1i1.570

ABSTRACTResearch about the effect of multiple doses of gamma irradiation to improved the appearance of the chrysanthemum plant has been carried out on chrysanthemum varieties Gold van Langen-doses of gamma irradiation: 0 Gy, 5 Gy, 10 Gy, and 15 Gy. Gamma irradiation has given at 2-week-old seedlings, which already have roots. Seeds that have been irradiated maintained up to 16 WAP. The results showed there are no all doses causes death in up to 16 WAP chrysanthemum seedlings but with a dose of 15 Gy seedlings did not grow at all. Dose of 5 Gy encourage the formation of lateral shoots on chrysanthemum. The higher the dose given causing the growth of seedlings of chrysanthemum hampered.Keywords: Chrysanthemum, Mutation Induction, irradiation.

HOMOLOGI FUNGSI GEN KNAT1 ( Knotted 1– like Arabidopsis thaliana) PADA ANGGREK BULAN Phalaenopsis amabilis (L.) Bl. DENGAN MEDIATOR Agrobacterium tumefaciens Sulastri Isminingsih; Endang Semiarti; Aziz Purwantoro
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTTo understand the function of KNAT1 gene Arabidopsis in the forming and developing of Indonesian origin orchid shoot Phalaenopsis amabilis through transform p35S::KNAT1 and pGreen to protocorm like bodies (plb) of the orchid mediated by Agrobacterium tumafaciens LBA4404. The plb transformants were grown on New Phalaenopsis selection medium containing 5 mg/l BAP, 0.15 mg/l NAA, 15 mg.l Kanamycin and addition of 300 mg/l Cefotaxim to eliminate the overgrowth of Agrobacterium. The analysis of positive transformant use Polimerase Chain Reaction (PCR) with the specific oligonucleotide primers for KNAT1 gene: KNAT1F1R1 and universal primers for pGreen: 35SO and Tnos. The result shows that 3 shoots of 1850 transformants positively carry out the 35S::KNAT1 construct (frequency of transformation was 0.16 %) while 5 shoot of 1850 transformants also positively carry out the pGreen vector, with the frequency of transformation was 0.27 %. The phenotype analysis of 35S::KNAT1 transformants show multiplication on forming of the leaf from a plb to + 10 shoots and forming of the leaf shape which has terompet like shapes and rectangular shape.Key words: Arabidopsis, KNAT1 gene, Phalaenopsis amabilis, shoot

PENANGANAN MASALAH PERSAMPAHAN DAN LIMBAH CAIR DI PROPINSI BANTEN Fitria Riany Eris
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTSolid and liquid waste are important environmental problems that if not managed well, it great potential to reduce the quality of the environment and health. Efforts to overcome the solid and liquid waste requires an approach. The objective of this research is to know the handling of environmental issues, especially solid and liquid waste handlers in Banten Province and provides an alternative waste management in overcoming it. Research done by taking a secondary data from various institutions related to research such as this BAPEDAL Province Banten, the BPS Banten Province and library literature. Handling environmental issues in Banten Province is stressed to technology and financial approach rather than through a social approach, partnership, cultural, political, legal and institutional arrangements. In connection with this effort will need, a new approach and change the paradigm in the management of solid and liquid waste. For example, the management of solid waste is concentrated to handle waste only and it should be changed to penyadaran process community and in the case of waste management.Key words: Solid Waste, Liquid Waste

SIFAT KIMIA ULTISOLS BANTEN AKIBAT PENGOLAHAN TANAH DAN PEMBERIAN PUPUK KOMPOS Dewi Firnia
Jurnal Agroekoteknologi Vol 1, No 1 (2009)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

ABSTRACTThe objective of this experiment were find out interaction effect between soil tillage and Compost dose to some chemistry properties of Ultisols Banten . The experiment was carried at the Kampung Baru Petir Serang Banten from June to September 2008, by using Randomized Block Design with factorial pattern and three replications. The first factor was soil tillage (one, two and three time tillage), and the second factor was compost dung (0; 10; 20; and ton ha-1). The data of experiment were analyzed by Fisher test and Duncan multiple range test =0,05 The result of experiment showed there was interaction effect between soil tillage and compost dosages only on the soil pH, but independently soil tillage and compost dosages application gave significant effect chemical properties ( C-Organic, and Nitrogen).Key words: Soil Tillage, Compost dung, Ultisols

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